Packaging and storage—
Preserve in tight containers, in a cool place.
Labeling—
Label it to indicate the names and proportions of any added stabilizers.
Iodine value, Method I 
401
:
between 23 and 40, about 550 mg of Polyoxyl 10 Oleyl Ether, accurately weighed, being used, and the reaction time being extended to 60 minutes.
Residue on ignition—
Weigh accurately about 25 g into a tared 40-mL porcelain crucible, and heat in contact with air until it ignites spontaneously, or can be ignited with a glowing splint. Allow the flame to go out, place the crucible in a muffle furnace with the door partly open until the carbon is consumed, close the door, and heat at about 700 ± 100
for 1 hour. Cool in a desiccator, weigh, and calculate the percentage of residue. If the amount so obtained exceeds 0.4%, again heat until constant weight is attained: the limit is 0.4%.
Free polyethylene glycols—
Transfer about 12 g, accurately weighed, to a 500-mL separator containing 50 mL of ethyl acetate. Add 50 mL of sodium chloride solution (29 in 100), shake vigorously for 2 minutes, and allow to separate for 15 minutes. Drain the lower, aqueous phase into a second 500-mL separator, and extract the upper layer with a second 50-mL portion of sodium chloride solution (29 in 100). To the combined aqueous layers add 50 mL of ethyl acetate, shake vigorously for 2 minutes, and allow to separate as before. Drain the lower, aqueous phase into a third 500-mL separator, and extract with two 50-mL portions of chloroform by shaking for 2 minutes each time. Evaporate the combined chloroform extracts in a 150-mL beaker on a steam bath, with the aid of a stream of nitrogen, to apparent dryness. Redissolve in about 15 mL of chloroform, and transfer to a filter, collecting the filtrate in a 150-mL beaker. Rinse the funnel with several small portions of chloroform, and evaporate the combined filtrate and rinsings, as described above, until no odor of chloroform or ethyl acetate is perceptible. Cool in a desiccator, and weigh: the limit is 7.5%.
Free ethylene oxide—
Internal standard solution—
Prepare a solution containing 100 mg of n-butyl chloride in each mL of chlorobenzene. Store in a tightly closed container. Prepare fresh weekly.
Standard solution—
[Caution—Ethylene oxide is toxic and flammable. Prepare this solution in a well-ventilated hood, using great care.
] Place 250 mL of chlorobenzene in a glass-stoppered, 500-mL conical flask. Bubble ethylene oxide through the chlorobenzene at a moderate rate for about 30 minutes, insert the stopper, and store with protection from heat. Pipet 25 mL of a 0.5 N alcoholic hydrochloric acid solution, prepared by mixing 45 mL of hydrochloric acid with 1 L of alcohol, into a 500-mL conical flask containing 40 g of magnesium chloride hexahydrate. Shake the mixture to effect saturation. Pipet 10 mL of the ethylene oxide solution into the flask, and add 20 drops of
bromocresol green TS. If the solution is not yellow (acid) at this point, add an additional volume, accurately measured, of 0.5 N alcoholic hydrochloric acid to give an excess of about 10 mL. Record the total volume of 0.5 N alcoholic hydrochloric acid added. Insert the stopper in the flask, and allow to stand for 30 minutes. Titrate the excess acid with 0.5 N alcoholic potassium hydroxide VS. Perform a blank titration, using 10.0 mL of chlorobenzene instead of ethylene oxide solution, adding the same total volume of 0.5 N alcoholic hydrochloric acid, and note the difference in volumes required. Each mL of the difference in volumes of 0.5 N alcoholic potassium hydroxide consumed is equivalent to 22.02 mg of ethylene oxide. Calculate the concentration, in mg per mL, of ethylene oxide in the
Standard solution. Standardize daily.
Standard preparation—
Transfer about 5 g of
USP Polyoxyl 10 Oleyl Ether RS to a suitable glass bottle of about 60-mL capacity, and add 10 mL of chlorobenzene, exactly 50 µL of
Internal standard solution, and an accurately measured volume of
Standard solution containing about 0.5 mg of ethylene oxide. Insert a magnetic stirring bar, cap the bottle tightly, and stir until homogeneity is attained.
Test preparation—
Transfer about 5 g of Polyoxyl 10 Oleyl Ether, accurately weighed, to a suitable glass bottle of about 60-mL capacity, and add 10 mL of chlorobenzene and 50 µL, accurately measured, of Internal standard solution. Insert a magnetic stirring bar, cap the bottle tightly, and stir until homogeneity is attained.
Chromatographic system—
Under typical conditions, the instrument is equipped with a flame-ionization detector, and contains a 1.8-m × 3-mm (OD) stainless steel column packed with S3. The injector port and detector block are maintained at about 210
and 230
, respectively, and the column at about 160
. Helium is used as the carrier gas at a flow rate of 66 mL per minute.
Interference check—
Inject a suitable volume of chlorobenzene into the gas chromatograph, and allow the chromatogram to run until the solvent has eluted. Similarly inject and chromatograph the Internal standard solution, the Standard solution, and a solution prepared according to the directions for the Test preparation, but omitting the internal standard. No interfering peaks are observed.
Procedure—
Inject about 2 µL of the
Standard preparation into a suitable gas chromatograph, and record the chromatogram. Similarly, inject about 2 µL of the
Test preparation, and record the chromatogram. Calculate the quantity, in mg, of ethylene oxide in the portion of Polyoxyl 10 Oleyl Ether taken by the formula:
WS(RU / RS),
in which
WS is the weight, in mg, of ethylene oxide in the portion of
Standard solution taken, and
RU and
RS are the area ratios of ethylene oxide to internal standard in the chromatograms for the
Test preparation and the
Standard preparation, respectively. The limit is 0.01%.
Average polymer length—
If solid material is present, place the Polyoxyl 10 Oleyl Ether in a 60
water bath overnight. Shake vigorously to eliminate any possibility of molecular weight gradients within it. Add about 1 mL of the melt to 1 mL of deuterated chloroform in a test tube, and shake the test tube until dissolution is complete. Transfer about 0.5 mL to an NMR tube, and add 5 drops of tetramethylsilane as an internal reference standard. Cap the tube tightly, and shake thoroughly. Place the tube in the NMR spectrometer, and record the NMR spectrum at an appropriate RF power level and a sweep time of 250 seconds per 500 Hz (see
Qualitative Scans under
Nuclear Magnetic Resonance 761). Adjust the spectrum amplitude so that the signal at 1.1 ppm is at least 80% of full scale. Record the integral areas from 0.4 ppm to 2.35 ppm (
A1), and from 2.35 ppm to 4.9 ppm (
A2) at a sweep time of 50 seconds per 500 Hz at an integral power level such that the integral of the largest peak is at least 80% of full chart height. Do not change the power level during the sweep. Record the integral of each peak several times, and calculate the average integral area. Calculate the number of oxyethylene units,
n, per molecule taken by the formula:
n = (31
A2 /
A1 
3) / 4,
in which 31 is the total number of protons in the molecule not activated by either oxygen or a double bond, 3 is the number of oxygen-activated protons not included in the oxyethylene unit count, and 4 is the number of protons in each oxyethylene unit.
-(Z)-9-octadecenyl-
-hydroxy-.
