A: Infrared Absorption 197M.

B: Ultraviolet Absorption 197U—

Test solution: 5 mg per mL, in Diluent, with the aid of sonication if necessary. Prepare the Diluent as follows.

Solution A— Dissolve 20.0 g of anhydrous sodium acetate in about 150 mL of water in a 250-mL volumetric flask. Add 50.0 mL of glacial acetic acid, dilute with water to volume, and mix.

Solution B— Dissolve 62.5 g of cupric sulfate in water in a 200-mL volumetric flask, dilute with water to volume, and mix.

Diluent— Mix Solution A and Solution B in a 1000-mL volumetric flask, dilute with water to volume, and mix.

Standard solutions— Dissolve USP Metyrosine RS in a solvent mixture of methanol and ammonium hydroxide (7:3) to obtain a solution having a concentration of 10 mg per mL (Standard solution A). Pipet 1 mL of Standard solution A into a 100-mL volumetric flask, dilute with the same solvent mixture to volume, and mix (Standard solution B). Pipet 5 mL of Standard solution B into a 10-mL volumetric flask, dilute with the same solvent mixture to volume, and mix (Standard solution C). Pipet 5 mL of Standard solution C into a 10-mL volumetric flask, dilute with the same solvent mixture to volume, and mix (Standard solution D).

Test solution— Dissolve Metyrosine in the solvent mixture of methanol and ammonium hydroxide (7:3) to obtain a solution having a concentration of 10 mg per mL.

Procedure— Apply 10-µL portions of Standard solutions A, B, C, and D and the Test solution to a suitable thin-layer chromatographic plate (see Chromatography 621) coated with a 0.25-mm layer of chromatographic silica gel mixture and previously washed with methanol. Allow the spots to dry, and develop the chromatogram in a solvent system consisting of a mixture of n-propyl alcohol and ammonium hydroxide (7:3) until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the developing chamber, mark the solvent front, and dry the plate. Expose the plate to iodine vapors, and examine under short-wavelength UV light: the chromatogram shows principal spots at about the same RF value. Estimate the levels of any additional spots observed in the chromatogram of the Test solution by comparison with the spots in the chromatograms of Standard solutions B, C, and D: the sum of the intensities of any spots observed is not greater than that of the principal spot obtained from Standard solution B, corresponding to not more than 1%.

(Official January 1, 2007)