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621
), coated with a 0.25-mm layer of chromatographic siliceous earth, by placing it in a developing chamber containing and equilibrated with a mixture of chloroform and corn oil (90:10), and allowing the solvent front to move about three-fourths of the length of the plate. Remove the plate, and allow the chloroform to evaporate. Apply 10 µL each of the solution under test and of a solution of USP Testosterone Cypionate RS in chloroform containing about 400 µg per mL on the plate, on a line about 2.5 cm from the bottom edge and about 1.5 cm apart. Place the plate in a developing chamber that contains and has been equilibrated with a mixture of methanol and water (90:10) previously saturated with corn oil. Develop the chromatogram until the solvent front has moved to about 10 cm above the line of application. Remove the plate, and heat in an oven at 105
for a few minutes. Spray the plate with a mixture of alcohol and sulfuric acid (3:1), and heat in an oven at 105 for 1 to 2 minutes. Observe the plate under long-wavelength UV light: the RF value of the principal spot obtained from the solution under test corresponds to that obtained from the Standard solution.
467:
meets the requirements.
1.
8. Remove the flask from the freezer, and immediately filter a portion of the contents. Allow the filtrate to reach room temperature, transfer a portion of it, equivalent to about 3 mg of testosterone cypionate, to a suitable vial, and evaporate to dryness. Add by pipet 3 mL of Internal standard solution, and shake vigorously to dissolve the residue.