Packaging and storage—
Preserve in well-closed containers. No storage requirements specified.
Identification—
A:
Under a microscope, using a mixture of glycerin and water (1:1) as a mounting agent, it presents granules, either irregularly shaped, ovoid, or pear-shaped, usually 30 µm to 100 µm in size, but occasionally exceeding 100 µm, or rounded, 10 µm to 35 µm in size. There are occasional compound granules having two or four components. The ovoid and pear-shaped granules have an eccentric hilum and the rounded granules an acentric or slightly eccentric hilum. All granules show clearly visible concentric striations. Between crossed nicol prisms, the granules show a distinct black cross intersecting at the hilum.
B:
Suspend 1 g of it in 50 mL of water, boil for 1 minute, and cool: a thin, cloudy mucilage is formed.
C:
To 1 mL of the mucilage obtained in Identification test B, add 0.05 mL of iodine and potassium iodide TS 2: an orange-red to dark blue color is produced, which disappears on heating.
Microbial limits 
61
—
The total aerobic microbial count does not exceed 1000 cfu per g, the total combined molds and yeasts count does not exceed 100 cfu per g, and it meets the requirements of the test for the absence of
Escherichia coli.
pH 791—
Prepare a slurry by weighing 5.0 g of Potato Starch, transferring to a suitable nonmetallic container, and adding 25.0 mL of freshly boiled and cooled water. Agitate continuously at a moderate rate for 1 minute. Stop the agitation, and allow to stand for 15 minutes. Determine the pH to the nearest 0.1 unit: the pH, determined potentiometrically, is between 5.0 and 8.0.
Loss on drying 731—
Dry about 1 g, accurately weighed, at 130
for 90 minutes: it loses not more than 20.0% of its weight.
Limit of iron—
Shake 1.5 g of Potato Starch with 15 mL of 2 N hydrochloric acid, and filter. Transfer 10 mL of the filtrate to a test tube, add 2 mL of citric acid solution (2 in 10) and 0.1 mL of thioglycolic acid, and mix. Add 10 N ammonium hydroxide until the solution is distinctly alkaline to litmus, dilute with water to 20 mL, and mix
(Test Solution). Prepare a
Standard Iron Solution containing the equivalent of 10 µg of iron per mL as directed under
Iron 241. Immediately before use, quantitatively dilute an accurately measured volume of this solution with water to obtain a diluted
Standard Iron Solution containing the equivalent of 1 µg of iron per mL. Prepare the
Standard Solution by transferring 10 mL of the diluted
Standard Iron Solution to a test tube and proceeding in the same manner as directed for the preparation of the
Test Solution, beginning with “add 2 mL of citric acid solution (2 in 10).” After 5 minutes, any pink color in the
Test Solution is not more intense than that in the
Standard Solution, corresponding to a limit of 10 µg of iron per g.
Limit of oxidizing substances—
Transfer 4.0 g to a glass-stoppered, 125-mL conical flask, and add 50.0 mL of water. Insert the stopper, and swirl for 5 minutes. Transfer to a glass-stoppered, 50-mL centrifuge tube, and centrifuge to clarify. Transfer 30.0 mL of the clear supernatant to a glass-stoppered, 125-mL conical flask. Add 1 mL of glacial acetic acid and 0.5 to 1.0 g of potassium iodide. Insert the stopper, swirl, and allow to stand for 25 to 30 minutes in the dark. Add 1 mL of starch TS, and titrate with 0.002 N sodium thiosulfate VS to the disappearance of the starch–iodine color. Perform a blank determination, and make any necessary correction. Each mL of 0.002 N sodium thiosulfate VS is equivalent to 34 µg of oxidant, calculated as hydrogen peroxide. Not more than 1.4 mL of 0.002 N sodium thiosulfate VS is required (20 µg per g, calculated as H2O2).
Limit of sulfur dioxide:
not more than 50 µg per g.
Reagents—
Carbon dioxide—
Use carbon dioxide, with a flow regulator that will maintain a flow of 100 ± 10 mL per minute.
Bromophenol blue indicator solution—
Dissolve 100 mg of bromophenol blue in 100 mL of dilute alcohol (1 in 5), and filter if necessary.
Hydrogen peroxide solution—
Dilute 30 percent hydrogen peroxide with water to obtain a 3% solution. Just before use, add 3 drops of Bromophenol blue indicator solution, and neutralize to a violet-blue endpoint with 0.01 N sodium hydroxide. Do not exceed the endpoint.
Apparatus—
In this test, the sulfur dioxide is released from the test specimen in a boiling acid medium and is removed by a stream of carbon dioxide. The separated gas is collected in a dilute hydrogen peroxide solution where the sulfur dioxide is oxidized to sulfuric acid and titrated with standard alkali. The apparatus consists essentially of a 500-mL three-neck, round-bottom boiling flask, a separatory funnel having a capacity of 100 mL or greater, a gas inlet tube of sufficient length to permit introduction of the carbon dioxide within 2.5 cm of the bottom of the boiling flask, a reflux condenser having a jacket length of 200 mm, and a delivery tube connecting the upper end of the reflux condenser to the bottom of a receiving test tube. Apply a thin film of stopcock grease to the sealing surfaces of all the joints except the joint between the separatory funnel and the boiling flask, and clamp the joints to ensure tightness.
Procedure—
Add 150 mL of water to the boiling flask. Close the stopcock of the separatory funnel, and begin the flow of carbon dioxide at a rate of 100 ± 5 mL per minute through the
Apparatus. Start the condenser coolant flow. Add 10 mL of
Hydrogen peroxide solution to a receiving test tube. After 15 minutes, without interrupting the flow of carbon dioxide, remove the separatory funnel from the boiling flask, and transfer 25.0 g of test specimen into the boiling flask with the aid of 100 mL of water. Apply stopcock grease to the outer joint of the separatory funnel, and replace the separatory funnel in the boiling flask. Close the stopcock of the separatory funnel, and add 80 mL of 2 N hydrochloric acid to the separatory funnel. Open the stopcock of the separatory funnel to permit the hydrochloric acid solution to flow into the boiling flask, guarding against the escape of sulfur dioxide into the separatory funnel by closing the stopcock before the last few mL of hydrochloric acid drain out. Boil the mixture for 1 hour. Remove the receiving test tube, and transfer its contents to a 200-mL wide-necked, conical flask. Rinse the receiving test tube with a small portion of water, add the rinsing to the 200-mL conical flask, and mix. Heat on a water bath for 15 minutes, and allow to cool. Add 0.1 mL of
Bromophenol blue indicator solution, and titrate the contents with 0.1 N sodium hydroxide VS until the color changes from yellow to violet-blue, with the color change lasting for at least 20 seconds. Perform a blank determination, and make any necessary correction (see
Titrimetry 541). Calculate the content, in µg per g, of sulfur dioxide in the test specimen taken by the formula:
1000(32.03)VN/W,
in which 32.03 is the milliequivalent weight of sulfur dioxide;
V is the volume, in mL, of titrant consumed;
N is the normality of the titrant; and
W is the weight, in g, of test specimen taken.
Residual solvents 467:
meets the requirements.
(Official January 1, 2007)
