Identification—
A:
The retention time of the major peak in the chromatogram of Test solution 1 corresponds to that in the chromatogram of the Reference solution, as obtained in the test for Chromatographic purity.
B:
Proceed as directed below.
Methoxyphenylacetic reagent—
Dissolve 2.7 g of methoxyphenylacetic acid in 6 mL of 10% tetramethylammonium hydroxide solution in methanol, and add 20 mL of alcohol. Store in a polyethylene container.
Procedure—
Dissolve about 20 mg of Sodium Caprylate in 0.5 mL of water, add 1.5 mL of
Methoxyphenylacetic reagent, and cool in ice water for 30 minutes. A voluminous, white, crystalline precipitate is formed. Place in water at 20
, and stir for 5 minutes. The precipitate does not disappear. Add 1 mL of ammonia TS. The precipitate dissolves completely. Add 1 mL of ammonium carbonate solution (16 in 100): no precipitate is formed.
Heavy metals, Method II 
231
—
Dissolve 2.0 g of Sodium Caprylate in 10 mL of glacial acetic acid, add 10 mL of alcohol, and use 12 mL of the solution obtained as the
Test Preparation. Prepare the
Standard Preparation using 1 mL of
Standard Lead Solution and 9 mL of a mixture of glacial acetic acid and alcohol (1:1). The limit is 5 µg per g.
Chromatographic purity—
Reference solution—
Transfer 10 mg of
USP Caprylic Acid RS to a 10-mL volumetric flask, and dilute with ethyl acetate to volume.
Test solution 1—
Dissolve 116 mg of Sodium Caprylate in 5 mL of water, add 1 mL of dilute sulfuric acid (1 in 35), and extract with 10 mL of ethyl acetate. Separate the organic layer, and dry it over anhydrous sodium sulfate.
Test solution 2—
Dilute 1.0 mL of Test solution 1 with ethyl acetate to 100 mL, transfer 5.0 mL of the solution obtained, and dilute with ethyl acetate to 50 mL.
Chromatographic system (see Chromatography 621)—
The gas chromatograph is equipped with a flame-ionization detector, a split injection system with a split ratio of about 1:100, and a 0.25-mm × 30-m fused silica column coated with a 0.25-µm layer of phase G25. The carrier gas is helium, flowing at a rate of 1.5 mL per minute. The chromatograph is programmed as follows. Initially the temperature of the column is equilibrated at 100
, then 1 minute after the injection, the temperature is increased at a rate of 5
per minute to 220
, and maintained at 220
for another 10 minutes. The injection port temperature and the detector temperature are maintained at 250
. Chromatograph
Test solution 2, and record the peak responses as directed for
Procedure: the signal-to-noise ratio of the principal peak is not less than 5.
Procedure—
Separately inject equal volumes (about 1 µL) of the
Reference solution, Test solution 2, and
Test solution 1 into the chromatograph, record the chromatograms, and measure the responses for the major peaks. Disregard any peaks with an area less than half of the area of the principal peak from
Test solution 2, and any peak due to the solvent. Calculate the percentage of each impurity in the portion of Sodium Caprylate taken by the formula:
100(ri / rs),
in which
ri is the peak response of the individual impurity, and
rs is the sum of the responses of all the peaks: not more than 0.3% of any impurity is found, and the sum of all the impurities found is not greater than 0.5%.
;)
