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USP Methyl Caprate RS. USP Methyl Caproate RS. USP Methyl Caprylate RS. USP Methyl Laurate RS. USP Methyl Linoleate RS. USP Methyl Linolenate RS. USP Methyl Myristate RS. USP Methyl Oleate RS. USP Methyl Palmitate RS. USP Methyl Palmitoleate RS. USP Methyl Stearate RS. USP 
-Sitosterol RS.
. Add 50 mL of a solution prepared by dissolving 130 g of potassium hydroxide in 200 mL of water and diluting with methanol to 1000 mL. Attach a condenser, and reflux in a bath at 100 until a clear solution is obtained. Reflux for an additional 10 minutes, and cool by adding 50 mL of water through the condenser. Quantitatively transfer to a separation funnel, rinsing the flask with a total of 50 mL of water in small portions. Extract with 80 mL of ether, shaking for 30 seconds, and repeat twice. [NOTE—If an emulsion forms, it can be eliminated by adding small quantities of methanol.] Transfer the combined ether layers to a separation funnel, and wash with successive portions of 50 mL of water until a neutral washing is obtained. [NOTE—If an emulsion forms, it can be eliminated by adding small quantities of methanol.] Pass the ether extract through filter paper containing anhydrous sodium sulfate, wash the filter with 30 mL of ether, and evaporate to dryness in vacuum. Dissolve the residue in 2.0 mL of chloroform.
-cholestanol in chloroform (1 in 100) to a thin-layer chromatographic plate coated with 0.25-mm silica gel having an application zone that was previously dipped under 3 cm of a solution prepared by dissolving 13 g of potassium hydroxide in 20 mL of water and diluted to 1000 mL with methanol. [NOTE—Allow the plate to dry, and heat it to 100 for 1 hour before use. The plate can be stored in a desiccator containing calcium chloride until the time of use.] Develop with a solvent consisting of a mixture of hexanes and ether (7:3) until the solvent front has moved 17 to 19 cm. Keep the chamber temperature between 15 and 20. Dry the plate in a current of warm air, then spray with an alkaline solution of 2,7-dichlorofluorescein in alcohol (0.2 in 100). Observe the plate under 366-nm wavelength light and identify the bands corresponding to the sterols by referring to the -cholestanol spot. Scrape off these bands and transfer them to a test tube. Add 10 mL of warm chloroform, and shake for 2 minutes with the aid of several glass beads. Filter the chloroform solution, wash the filter with chloroform, and evaporate the combined filtrate and washings to dryness in vacuum. Dissolve the residue with some drops of anhydrous acetone and evaporate in vacuum. Dry the residue in an oven at 105 for 15 minutes. Dissolve the residue in 0.2 mL of Derivatizing reagent, and allow to stand for not less than 15 minutes at room temperature.-sitosterol, and stigmasterol, identified by their retention times relative to the -sitosterol peak in the chromatogram of the Standard solution.
2021—
Capsules meet the requirements of the tests for absence of Salmonella species, Escherichia coli, and Staphylococcus aureus. The total bacterial count does not exceed 10,000 cfu per g, the total combined molds and yeasts count does not exceed 1000 cfu per g, the coliform count does not exceed 100 cfu per g, and the count for enterobacteria does not exceed 100 cfu per g.
2040).
2091:
meet the requirements.
for 2 hours, shaking from time to time. Allow to cool, and add 1.0 mL of Internal standard solution, 10.0 mL of water, 1 g of sodium chloride, and 5 mL of hexanes. Shake well, and allow the layers to separate completely. Use the hexanes layer. [NOTE—Store this solution in a refrigerator until use.]
467:
meet the requirements.