C4H10N2 86.14

Piperazine.
Piperazine [110-85-0].

Packaging and storage— Preserve in tight containers, protected from light.

Color of solution— Dissolve 10.0 g in water, and dilute with water to 50.0 mL: the solution has no more color than a standard solution prepared by adding 2.0 mL of ferric chloride CS to water and diluting with water to 50.0 mL, when compared in matched color-comparison tubes.

Identification— It responds to Identification test A under Piperazine Citrate.

Melting range 741: between 109 and 113.

Water, Method I 921: not more than 2.0%.

Primary amines and ammonia— Dissolve 200 mg in 10 mL of water, add 1 mL of acetone and 0.5 mL of freshly prepared sodium nitroferricyanide solution (1 in 10), mix, and allow to stand for 10 minutes, accurately timed. Determine the absorbance of this solution at 520 nm and at 600 nm, using a reagent blank as the reference solution. The ratio A600/A520 is not more than 0.5 (equivalent to about 0.7% of primary amines and ammonia).

Residual solvents 467: meets the requirements.

Assay— Weigh accurately about 150 mg of Piperazine, and dissolve in 75 mL of glacial acetic acid. Titrate potentiometrically with 0.1 N perchloric acid VS, using a silver-glass electrode system. As the endpoint is approached, warm the solution to 60 to 70, then complete the titration. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 4.307 mg of C4H10N2.

Expert Committee : (MDAA05) Monograph Development-Antivirals and Antimicrobials

USP29–NF24 Page 1741

Phone Number : 1-301-816-8394