Identification—
Transfer a volume of Injection, equivalent to about 2 mg of deslanoside, to a small separator, and extract with 25 mL of a mixture of 7 volumes of chloroform and 3 volumes of alcohol. Transfer the extract to a 10-mL conical flask, evaporate on a steam bath to dryness, and dissolve the residue in 500 µL of methanol. Proceed with a 5-µL portion of the solution so obtained, as directed in the
Identification test under
Deslanoside, beginning with “Apply 5 µL of this solution.” The specified result is obtained.
Assay—
Assay preparation—
Transfer a volume of Injection, equivalent to about 600 µg of deslanoside, to a separator, and add 50 mL of water and 1 mL of 2 N sulfuric acid. Extract with four 30-mL portions of a mixture of 5 volumes of chloroform and 1 volume of n-propyl alcohol, washing each portion in a second separator containing 5 mL of water and filtering through cotton that previously has been moistened with chloroform. Combine the extracts, and evaporate on a steam bath, with the aid of a current of air, just to dryness. Transfer the residue, with the aid of a small volume of the chloroform–n-propyl alcohol mixture, to a 25-mL conical flask.
Procedure—
Transfer 3.0 mL each of the
Standard preparation and of alcohol to provide the blank to separate 25-mL conical flasks. Using these solutions and the
Assay preparation, proceed as directed for
Procedure in the
Assay under
Deslanoside, beginning with “Evaporate each with gentle warming.” Calculate the quantity, in µg, of C
47H
74O
19 in each mL of the Injection taken by the formula:
(C / V)(AU / AS),
in which
C is the concentration, in µg per mL, of
USP Deslanoside RS in the
Standard preparation; V is the volume, in mL, of Injection taken; and
AU and
AS are the maximum absorbances of the solutions from the
Assay preparation and the
Standard preparation, respectively.
791
:
between 5.5 and 7.0.