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197K
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Obtain the test specimen as follows. Transfer a quantity of Intramammary Infusion, equivalent to about 500 mg of cloxacillin, to a 50-mL centrifuge tube, add 25 mL of toluene, mix, and centrifuge. Decant and discard the toluene. Wash the residue with four 25-mL portions of toluene, sonicating for about 30 seconds after each addition of toluene. Dry the residue in vacuum over silica gel.
71 (where labeled as being sterile)—
It meets the requirements when tested as directed for Direct Inoculation of the Culture Medium under Test for Sterility of the Product to be Examined, except to use Fluid Thioglycollate Medium containing polysorbate 80 solution (1 in 200) and an amount of sterile penicillinase sufficient to inactivate the cloxacillin in each tube, to use Soybean–Casein Digest Medium containing polysorbate 80 solution (1 in 200) and an amount of sterile penicillinase sufficient to inactivate the cloxacillin in each tube, and to shake the tubes once daily.
921:
not more than 1.0%, 20 mL of a mixture of toluene and methanol (7:3) being used in place of methanol in the titration vessel.
467:
meets the requirements.
81, expelling the contents of 1 syringe of Intramammary Infusion into a high-speed glass blender jar containing sufficient methanol to yield a volume of 500 mL, and blend for 3 to 5 minutes. Promptly dilute an accurately measured volume of this solution quantitatively and stepwise with Buffer No. 1 to obtain a Test Dilution having a concentration assumed to be equal to the median dose level of the Standard.