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C4H4N2O2—112.09—White to cream-colored, crystalline powder. Melts above 300
. One g dissolves in about 500 mL of water; less soluble in alcohol; soluble in ammonia TS and in sodium hydroxide TS. Its solutions yield no precipitate with the usual alkaloidal precipitants.
negligible, from 100 mg.
731
—
Dry it at 105 for 2 hours: it loses not more than 2% of its weight.
UO2(C2H3O2)2·2H2O—424.15—Use ACS reagent grade.
NH2CONH2—60.06—Use ACS reagent grade.
C3H7NO2—89.09—White powder with chunks. Freely soluble in water.
C9H12N2O6—244.20—White powder.
MOBILE PHASE—
Prepare a mixture of methanol and 0.2 M ammonium acetate (90:10), and adjust with phosphoric acid to a pH of 7.0.
TEST SOLUTION:
0.5 mg per mL.
PROCEDURE—
Inject about 20 µL of the Test solution into a liquid chromatograph (see Chromatography 621), equipped with a 280-nm detector and a 4.6-mm × 15-cm column that contains packing L1. The flow rate is about 2.0 mL per minute. The area of the C9H12N2O6 peak is not less than 99% of the total peak area.