NOTE—Throughout the following procedures, protect test or assay specimens, the Reference Standard, and solutions containing them, by conducting the procedures without delay, under subdued light, or by using low-actinic glassware.

A: Infrared Absorption 197M.

B: Ultraviolet Absorption 197U—

C: A solution (1 in 100) responds to the tests for Chloride 191.

D: Prepare a solution in methanol containing 1.2 mg per mL. Apply 5 µL each of this solution and a solution of USP Trifluoperazine Hydrochloride RS in methanol containing 1.2 mg per mL to a suitable thin-layer chromatographic plate (see Chromatography 621) coated with a 0.25-mm layer of chromatographic silica gel. Allow the spots to dry, and develop the chromatogram in a solvent system consisting of a mixture of acetone and ammonium hydroxide (200:1) until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the developing chamber, mark the solvent front, and allow the solvent to evaporate. Locate the spots on the plate by lightly spraying with a solution of iodoplatinic acid prepared by dissolving 100 mg of chloroplatinic acid in 1 mL of 1 N hydrochloric acid, adding 25 mL of potassium iodide solution (1 in 25), diluting with water to 100 mL, and then adding 0.5 mL of formic acid: the RF value of the principal spot from the test solution corresponds to that from the Standard solution.

(Official January 1, 2007)