Identification
Shake a quantity equivalent to about 50 mg with 50 mL of water, and add 2.5 N sodium hydroxide dropwise until the solution becomes clear. Add diluted hydrochloric acid dropwise until a precipitate forms. Collect the precipitate on a very fine filter, and wash it with water and with ether: the sulfadimethoxine so obtained meets the requirements for the
Identification tests under
Sulfadimethoxine.
Assay
Mobile phase, Standard preparation, and Chromatographic system
Proceed as directed in the
Assay under
Sulfadimethoxine.
Assay preparation
Transfer an accurately measured volume of Suspension, previously mixed and free from air bubbles, equivalent to about 50 mg of sulfadimethoxine, to a 250-mL volumetric flask, add about 200 mL of Mobile phase, and swirl to dissolve. Dilute with Mobile phase to volume, and mix. Protect this solution from light.
Procedure
Proceed as directed in the
Assay under
Sulfadimethoxine. Calculate the quantity, in mg, of sulfadimethoxine (C
12H
14N
4O
4S) in each mL of the Oral Suspension taken by the formula:
250(C/V)(rU / rS),
in which
V is the volume, in mL, of Oral Suspension taken to prepare the
Assay preparation; and the other terms are as defined therein.