Assay
Diluent
Transfer 500.0 mL of a 4.0 M sodium hydroxide solution to a 1000-mL volumetric flask, and add about 67.0 g of edetate disodium and 36.0 g of ascorbic acid, each accurately weighed. Dilute with water to volume, and mix.
Assay preparation
Transfer an accurately weighed portion of the Topical Gel, equivalent to about 30 mg of sodium sulfide, to a 100-mL volumetric flask, add 50 mL of Diluent, and shake by mechanical means until the Topical Gel is dispersed. Dilute with Diluent to volume, and mix.
Procedure
Transfer 30.0 mL of the Assay preparation to a suitable beaker, and titrate with 0.01 M lead perchlorate VS, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.01 M lead perchlorate is equivalent to 2.4018 mg of sodium sulfide (Na2S·9H2O).