2(3
H)-Furanone, 3-ethyldihydro-4-[(1-methyl-1
H-imidazol-5-yl)methyl]-, monohydrochloride, (3
S-cis)-.
Pilocarpine monohydrochloride
[
54-71-7].
Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
B:
A solution (1 in 20) responds to the tests for
Chloride 
191
.
Melting range 741:
between 199
and 205
, but the range between beginning and end of melting does not exceed 3
.
Specific rotation 781S:
between +88.5
and +91.5
.
Test solution:
20 mg per mL, in water.
Ordinary impurities 466—
Test solution:
dehydrated alcohol.
Standard solution:
dehydrated alcohol.
Eluant:
a mixture of hexanes, dehydrated alcohol, and ammonium hydroxide (70:30:1).
Visualization:
17.
Limits:
not more than 1%.
Other alkaloids—
Dissolve 200 mg in 20 mL of water, and divide the solution into two portions. To one portion add a few drops of 6 N ammonium hydroxide, and to the other add a few drops of
potassium dichromate TS: no turbidity is produced in either solution.
Assay—
Dissolve about 500 mg of Pilocarpine Hydrochloride, accurately weighed, in a mixture of 20 mL of glacial acetic acid and 10 mL of
mercuric acetate TS, warming slightly to effect solution. Cool the solution to room temperature, add 2 drops of
crystal violet TS, and titrate with 0.1 N perchloric acid VS. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 24.47 mg of C
11H
16N
2O
2·HCl.
