Limit of hydroxylamine—
Phosphate buffer—
Dissolve 1.36 g of monobasic potassium phosphate in about 950 mL of water, adjust with 1 M potassium hydroxide to a pH of 7.4, dilute with water to 1000 mL, and mix.
Pyridoxal 5-phosphate solution—
Dissolve 50 mg of pyridoxal 5-phosphate monohydrate in 50 mL of Phosphate buffer in a low-actinic flask. Prepare fresh before use.
Standard solutions—
Dissolve an accurately weighed quantity of hydroxylamine hydrochloride in water to obtain a final concentration of 2.0 mg per mL. To separate 100-mL volumetric flasks, transfer 5.0, 10.0, and 15.0 mL of the hydroxylamine stock solution, respectively, dilute each flask with water to volume, and mix.
Test solution—
Transfer an accurately weighed quantity of about 1500 mg of Acetohydroxamic Acid, previously dried, to a 100-mL beaker, and dissolve in a sufficient amount of water to cover the electrode of a calibrated pH meter (about 60 mL). While stirring, adjust with 0.05 M potassium hydroxide to a pH of 7.4. Quantitatively transfer the contents of the beaker, with the aid of small portions of water, to a 100-mL volumetric flask, dilute with water to volume, and mix.
Procedure—
Transfer 2.0 mL of each
Standard solution and the
Test solution into separate 100-mL volumetric flasks. Pipet 2.0 mL of water into a 100-mL volumetric flask for the reagent blank. To each flask, add 4.0 mL of
Pyridoxal 5-phosphate solution, and mix. After 8 minutes, accurately timed, dilute the contents of each flask with
Phosphate buffer to volume. Immediately determine the fluorescence intensities of the solutions from the
Standard solutions and the
Test solution in a fluorometer at an excitation wavelength of 350 nm and an emission wavelength of 450 nm, setting the instrument to zero with the reagent blank. Determine the best-fit straight line from the fluorescence intensities of the three
Standard solutions versus the hydroxylamine hydrochloride concentrations, in µg per mL. From the best-fit straight line, determine the concentration, in µg per mL, of hydroxylamine hydrochloride in the
Test solution. Calculate the percentage of hydroxylamine in the portion of Acetohydroxamic Acid taken by the formula:
(33.03/69.50)(10C/W),
in which 33.03 and 69.50 are the molecular weights of hydroxylamine and hydroxylamine hydrochloride, respectively;
C is the concentration, in µg per mL, of hydroxylamine hydrochloride in the
Test solution; and
W is the weight, in mg, of Acetohydroxamic Acid taken. Not more than 0.5% is found.
Assay—
Ferric chloride solution—
Dissolve 4 g of ferric chloride in about 50 mL of 0.1 N hydrochloric acid, dilute with the same solvent to make 200 mL, and mix.
Standard preparation—
Dissolve an accurately weighed quantity of
USP Acetohydroxamic Acid RS in 0.1 N hydrochloric acid to obtain a solution having a known concentration of about 500 µg per mL.
Assay preparation—
Transfer about 250 mg of Acetohydroxamic Acid, previously dried and accurately weighed, to a 500-mL volumetric flask, dilute with 0.1 N hydrochloric acid to volume, and mix.
Procedure—
Transfer 10.0 mL each of the
Standard preparation, the
Assay preparation, and 0.1 N hydrochloric acid to provide the blank, to separate 100-mL volumetric flasks. To each flask add about 50 mL of 0.1 N hydrochloric acid and 10.0 mL of
Ferric chloride solution, mix, dilute with 0.1 N hydrochloric acid to volume, and mix. Without delay, concomitantly determine the absorbances of the solutions from the
Standard preparation and the
Assay preparation at the wavelength of maximum absorbance at about 502 nm, with a suitable spectrophotometer, using the blank to set the instrument. Calculate the quantity, in mg, of C
2H
5NO
2 in the portion of Acetohydroxamic Acid taken by the formula:
0.5C(AU / AS),
in which
C is the concentration, in µg per mL, of
USP Acetohydroxamic Acid RS in the
Standard preparation; and
AU and
AS are the absorbances of the
Assay preparation and the
Standard preparation, respectively.
;)
641
—
A 1.0-g portion dissolves in 10 mL of water to yield a clear solution.