1-Propanaminium, 2-(acetyloxy)-
N,
N,
N-trimethyl-, chloride, (±)-.
(±)-(2-Hydroxypropyl)trimethylammonium chloride acetate
[
62-51-1].
Packaging and storage—
Preserve in tight containers.
Identification—
A:
Dissolve about 100 mg in about 2 mL of water on a watch glass, and add 3 mL of
platinic chloride TS: small rhombohedric plates are formed, which melt between 220
and 225
(see
Melting Range or Temperature 
741
) (
distinction from acetylcholine chloride,
which forms needles radiating from a central point,
and from choline chloride,
which forms no crystals).
B:
To 1 mL of a solution (1 in 10) add 1 mL of alcohol and 1 mL of sulfuric acid, and heat gently: the odor of ethyl acetate is perceptible.
C:
To 5 mL of a solution (1 in 10) add 2 g of potassium hydroxide and heat gently: the odor of trimethylamine is perceptible.
D:
A solution (1 in 50) responds to the tests for
Chloride 191.
Melting range 741—
Dissolve about 100 mg in 2 to 3 mL of chloroform in a small beaker. Heat at 110
for 1 hour. While the test specimen is still hot, quickly powder the dry residue with a glass rod, and transfer to a melting point tube in the usual manner. Determine the melting range without delay. It melts between 170
and 173
.
Acetylcholine chloride—
To 2 mL of a solution (1 in 10) add 3 mL of a solution of sodium perchlorate (1 in 5), shake, and immerse in ice water for 5 minutes: no precipitate is formed.
Heavy metals, Method II 231:
0.002%.
Assay—
Transfer to a conical flask about 400 mg of Methacholine Chloride, previously dried and accurately weighed (because it is very hygroscopic, store the dried material in a vacuum desiccator), dissolve it in 50 mL of glacial acetic acid, add 10 mL of
mercuric acetate TS and 1 drop of
crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue-green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 19.57 mg of C
8H
18ClNO
2.
;)