Identification—
Extract a volume of Injectable Suspension, equivalent to about 50 mg of hydrocortisone acetate, with two 10-mL portions of peroxide-free ether, and discard the ether extracts. Filter with suction, wash with small portions of water, and dry at 105
for 1 hour: the hydrocortisone acetate so obtained responds to
Identification test
A under
Hydrocortisone Acetate.
Assay—
Assay preparation—
Transfer to a separator an accurately measured volume of Injectable Suspension, equivalent to about 50 mg of hydrocortisone acetate, and dilute with water to about 15 mL. Extract with four 25-mL portions of chloroform, filtering each portion through chloroform-washed cotton into a 250-mL volumetric flask. Add chloroform to volume, and mix. Pipet 10 mL of this solution into a 100-mL volumetric flask, add chloroform to volume, and mix. Pipet 10 mL of the resulting solution into a glass-stoppered, 50-mL conical flask, evaporate the chloroform on a steam bath just to dryness, cool, and dissolve the residue in 20.0 mL of alcohol.
Procedure—
Proceed as directed for
Procedure under
Assay for Steroids 
351
. Calculate the quantity, in mg, of hydrocortisone acetate (C
23H
32O
6) in each mL of the Injectable Suspension taken by the formula:
5(C / V)(AU / AS),
in which
V is the volume, in mL, of Injectable Suspension taken; and the other terms are as defined therein.
