C2H5NO2 75.07

Glycine.
Glycine [56-40-6].

Packaging and storage— Preserve in well-closed containers.

USP Reference standards 11— USP Glycine RS.

Identification, Infrared Absorption 197M.

Loss on drying 731— Dry it at 105 for 2 hours: it loses not more than 0.2% of its weight.

Residue on ignition 281: not more than 0.1%.

Chloride 221— A solution of 1.0 g in water shows no more chloride than corresponds to 0.10 mL of 0.020 N hydrochloric acid (0.007%).

Sulfate 221— A solution of 3.0 g in water shows no more sulfate than corresponds to 0.20 mL of 0.020 N sulfuric acid (0.0065%).

Heavy metals 231: 0.002%.

Hydrolyzable substances— Boil 10 mL of a 1 in 10 solution of it for 1 minute, and set aside for 2 hours: the solution appears as clear and as mobile as 10 mL of the same solution that has not been boiled.

Organic volatile impurities, Method I 467: meets the requirements.

Residual solvents 467: meets the requirements.

Assay— Transfer about 150 mg of Glycine, accurately weighed, to a 250-mL flask, dissolve in 100 mL of glacial acetic acid, add 1 drop of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 7.507 mg of C2H5NO2.

Expert Committee : (DSN05) Dietary Supplements - Non-Botanicals

USP29–NF24 Page 1013

Phone Number : 1-301-816-8389