Identification
A:
Dissolve about 50 mg in 5 mL of water, render the solution alkaline with 6 N ammonium hydroxide, and extract with two 10-mL portions of ether. Evaporate the ether on a steam bath, and recrystallize the residue from solvent hexane: the eucatropine base so obtained melts between 111
and 114
.
B:
A solution of it responds to the tests for
Chloride 191.
Assay
Weigh accurately about 500 mg of Eucatropine Hydrochloride, and dissolve it in 10 mL of water. Saturate the solution with sodium chloride. Render it alkaline with 6 N ammonium hydroxide, and extract the base completely with successive 15-mL portions of ether. Wash the combined ether extracts with 10 mL of water, and extract the water washing with 10 mL of ether. To the combined ether solutions add 25.0 mL of 0.1 N sulfuric acid VS, and stir. Heat gently until the ether is expelled, cool, add
methyl red TS, and titrate the excess acid with 0.1 N sodium hydroxide VS. Each mL of 0.1 N sulfuric acid is equivalent to 32.79 mg of C
17H
25NO
3·HCl.