C2H8N2 60.10

1,2-Ethanediamine.
Ethylenediamine [107-15-3].

Packaging and storage— Preserve in well-filled, tight, glass containers.

Identification— To 2 mL of cupric sulfate solution (1 in 100) add 3 drops of a solution of Ethylenediamine (1 in 6), and shake: a purplish blue color is produced.

Heavy metals, Method I 231— Evaporate 5.0 mL on a steam bath to dryness, add to the residue 1 mL of hydrochloric acid and 0.5 mL of nitric acid, and evaporate again to dryness. Dissolve the residue in 20 mL of warm water, cool, dilute with water to 100 mL, mix, and use 20 mL of this solution for the test: the limit is 0.002%.

Organic volatile impurities, Method IV 467: meets the requirements.

Residual solvents 467: meets the requirements.

Assay— Weigh accurately about 1 mL of Ethylenediamine in a tared, glass-stoppered flask containing about 25 mL of water. Dilute with water to about 75 mL, add a mixed indicator of bromocresol green TS and methyl red TS (5 in 6), mix, and titrate with 1 N hydrochloric acid VS. Perform a blank determination, and make any necessary correction. Each mL of 1 N hydrochloric acid is equivalent to 30.05 mg of C2H8N2.

Expert Committee : (MDGRE05) Monograph Development-Gastrointestinal Renal and Endocrine

USP29–NF24 Page 870

Phone Number : 1-301-816-8251