Benzenemethanaminium,
N-[2-[(2,6-dimethylphenyl)amino]-2-oxoethyl]-
N,
N-diethyl-, benzoate, monohydrate.
Benzyldiethyl[(2,6-xylylcarbamoyl)methyl]ammonium benzoate monohydrate
[
86398-53-0].
Anhydrous
446.59
[
3734-33-6].
Packaging and storage—
Preserve in tight containers.
Labeling—
Label it to indicate whether it is hydrous or anhydrous.
Identification—
Solution:
100 µg per mL.
Medium:
water.
Absorptivities at 263 nm, calculated on the dried basis, do not differ by more than 3.0%.
C:
Dissolve about 150 mg in 10 mL of water, and add 15 mL of trinitrophenol TS: a yellow precipitate is formed.
D:
Dissolve about 100 mg in 10 mL of water, and add 20 mL of 2 N sulfuric acid and 15 mL of
ammonium reineckate TS. Mix, filter through a sintered-glass crucible using gentle suction, and wash thoroughly with water. Remove as much water as possible with suction, and then dry in an oven at 105
for 1 hour: the denatonium reineckate so obtained melts at about 170
(see
Melting Range or Temperature 
741
).
pH 791:
between 6.5 and 7.5, in a solution (3 in 100).
Loss on drying 731—
Dry it at 105
for 2 hours: the monohydrate loses between 3.5% and 4.5% of its weight, and the anhydrous form loses not more than 1.0% of its weight.
Chloride 221—
Dissolve 350 mg in 9 mL of water, add 1 mL of nitric acid, and filter. A 1.0-mL portion of the filtrate shows no more chloride than corresponds to 0.10 mL of 0.020 N hydrochloric acid (0.2%).
Assay—
Dissolve about 900 mg of Denatonium Benzoate, previously dried and accurately weighed, in 50 mL of glacial acetic acid, add 1 drop of
crystal violet TS, and titrate with 0.1 N perchloric acid VS to a green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 44.66 mg of C
28H
34N
2O
3.
;)