Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
B:
Dissolve 500 mg in 10 mL of a mixture of 3 volumes of alcohol and 2 volumes of water, warming if necessary. Cool the solution in an ice bath, add 1 mL of 1 N sodium hydroxide and 20 mL of water, stir, and filter: the precipitate of the base so obtained, washed with water and dried in vacuum at 60
for 2 hours, melts between 106
and 109
.
C:
It responds to the tests for
Chloride 
191
.
pH 791:
between 4.5 and 5.5, determined potentiometrically in a 1 in 50 solution, the solvent being a mixture of 2 volumes of alcohol and 3 volumes of water.
Ordinary impurities 466—
Test solution:
methanol.
Standard solution:
methanol.
Eluant:
a mixture of chloroform, methanol, and ammonium hydroxide (80:20:1).
Visualization:
2.
Organic volatile impurities, Method IV 467:
meets the requirements.
Assay—
Transfer to a beaker about 400 mg of Cyclizine Hydrochloride, accurately weighed, and dissolve in 80 mL of glacial acetic acid. Add 10 mL of
mercuric acetate TS, and titrate with 0.1 N perchloric acid VS, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 15.14 mg of C
18H
22N
2·HCl.
