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Pipet 105 mL of water at 10 to 15 into a standard Bloom bottle, add 7.5 g of Gelatin, and stir. Allow to stand for 1 hour, then bring to a temperature of 62 in 15 minutes by placing in a water bath regulated at 65 (the substance may be swirled several times to aid solution). Finally mix by inversion, allow to stand for 15 minutes, and place in a water bath at 10 ± 0.1. Chill, without disturbance, for 17 hours. Determine the gel strength in a Bloom Gelometer (a device developed to make this determination under standardized conditions) adjusted for 4-mm depression and to deliver 200 ± 5 g of shot per 5 seconds, using the 12.7-mm diameter (nonbeveled) plunger.

Auxiliary Information—
Staff Liaison : Hong Wang, Ph.D. , Senior Scientific Associate
Expert Committee : (EGC05) Excipient General Chapters
USP29–NF24 Page 2920
Phone Number : 1-301-816-8351